Important note:
Please, note that there might be no HSPs accumulation below temperature of 32-34°C. HSPs are induced when the plant experience temperatures higher than the growing temperature with around 10°C. So, the HSPs induction temperatures for plants grown at 18C differ from these for plants grown at 24C. Another very effective parameter is the humidity. When using low humidity the plant has a chance to cool down through transpiration. In this case the HSPs induction requires higher temperatures.
Agrisera western blot recommendations and trouble shooting and Video tutorial can be found here.
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1 | PEB (4x) | protein extraction buffer
AS08 300 | extraction buffer for quantitative isolation of total soluble/membrane protein from plant tissue and algal/bacterial cells, optimized for subsequent western blot detection |
126 € |
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ATPase AAA2 domain
AS11 1754 | clonality: polyclonal | host: rabbit | reactivity:Arabidopsis thaliana, Synechococcus sp. PCC7942
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304 € |
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ClpB-P | ClpB3
AS09 459 | clonality:polyclonal | host:rabbit | reactivity: Arabidopsis thaliana |
304 € |
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HSP70B | stromal alfa-HSP70
AS06 175 | clonality: polyclonal | host: rabbit | reactivity: Ch. reinhardtii, D. subspicatus, P. patens
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304 € |
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