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product information
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| background |
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PFOR (pyruvate-ferredoxin oxidoreductase) is an enzyme which belongs to a family of oxidoreducatases and participates in 4 metabolic pathways: pyruvate metabolism, propanoate metabolism, butanoate metabolism and reductive carboxylate cycle (carbon dioxide fication). Alternative names: pyruvate oxidoreductase, pyruvate synthetase, pyruvic-ferredoxin oxidoreductase.
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| immunogen |
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KLH-conjugated synthetic peptide conserved in Chlamydomonas reinhardtii PFOR protein A8JEH2 |
| antibody format |
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rabbit |
polyclonal |
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affinity purified serum, in PBS pH 7.4, |
lyophilized |
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| quantity |
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200 µg |
for reconstitution add 200 µl, of sterile water. |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB) |
| related products |
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AS05 067 | Anti-POR | protochlorophilide oxidoreductase antibody
AS10 1625 | Anti-FNR | ferredoxin-NADP+-oxidoreductase antibody
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| additional information |
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to be added when available |
application information
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| recommended dilution |
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1: 1000 (WB) |
| expected | apparent MW |
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130 kDa |
| confirmed reactivity |
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Chlamydomonas reinhardtii |
| predicted reactivity |
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Volvox carteri |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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to be added when available |
| selected references |
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to be added when available, antibody released in April 2011 |
application example
Chlamydomonas reinhardtii total cell extract - oxic sample (1), and anoxia 2 h(2), 4h (3), 6h (4) 8h (5). Total proteins were extracted with 50 mM Tris buffer (pH 8.0) containing 10 mM EDTA and 2% SDS, 30ug of totale protein were separated by SDS-PAGE using a 10% polyacrylamide gel and then transferred 1h at RT to PVDF membranes. The membranes were blocked with a 5% milk in TBS-T. Blot was incubated in the primary anti-PFOR antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was seconds 5 min. Courtesty Dr. Caludia Catalanotti, Carnegie Institution, USA
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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