Bromelain, Biotin conjugated

315 €

AS09 553 | clonality: polyclonal | host: rabbit | reactivity: Ananas comosus

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product information

background

Bromelain (EC=3.4.22.32) is a cysteine protease isolated from pineapple stem.

immunogen

native bromelain isolated and purified from Ananas comosus

antibody format

rabbit

polyclonal

purified IgG in phosphate buffer saline,

lyophilized

quantity

1 ml	for reconstitution please add 1 ml of sterile destilled water.

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohitochemistry (IHC)

related products

AS09 552 | anti-bromelain

AS09 554 | anti-bromelain (affinity purified)

AS09 546 | anti-PPI | papain (biotinylated)

AS09 547 | anti-PPI | papain (affinity purified)

additional information

Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

application information
recommended dilution		1: 5000 - 1: 50 000 for techniques listed above
expected \| apparent MW		n.a.
confirmed reactivity		Ananas comosus
predicted reactivity		Ananas comosus
not reactive in		no confirmed exceptions from predicted reactivity known in the moment
additional information		Biotin/IgG protein molar ration is approximately 6.2. No foreign proteins are added.
selected references		to be added when available