Plant/Algal cell antibodies / Membrane transport system / Endomembrane system

product information

MTP4 protein is involved in maintaining zinc homeostasis by sequestration of excess zinc in the cytoplasm into vacuole. Alternative name: AtMTPb

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana  Q6DBM8

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

ELISA (ELISA), western blot (WB)

collection of antibodies to tonoplast proteins

AS09 485 | anti-AtMTP1 | vacuolar Zn2+/H+ antiporter

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few  µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Protocol for isolation of vacuolar membranes can be found here.

application information

1: 8000 (ELISA), 1: 2000 with standard ECL (WB)

42.3 | 43 kDa (Arabidopsis thaliana)

Arabidopsis thaliana

dicots including: Capsella rubella

no confirmed exceptions from predicted reactivity known in the moment

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it  contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

to be added when available

application example

5 µg of crude membrane fraction from Arabidopsis thaliana incubated with peptide used to elicit anti-AtMTP4 antibodies (1), 5 µg of crude membrane fraction from Arabidopsis thaliana (2), His tag recombinant MTP4 protein (3), were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-AJ011604 | nitrate transporter antibodies (AS09 474 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.