Plant/Algal cell antibodies / Membrane transport system / Endomembrane system

product information

HDEL (his-asp-glu-leu) is a C-terminal tertapeptide found in yeast and plants which allows sorting of resident soluble proteins in the lumen of the endoplasmic reticulum.

synthetic HDEL peptide corresponding to the C-terminus of yeast Bip

store at -20°C for 1 year; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB), immunofluorescence (IF), immunogold (IG)

AS10 683-25 | HDEL | endoplasmic reticulum retention signal mouse antibody, 25 µg

collection of antibodies for endomembrane proteins

protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 1 mg/ml

application information

1: 100 - 1. 1000  (WB), 1. 50- 1: 500 (IF)

78 kDa

Barnyard Grass (E. crus-galli), B. vulgaris, G. hirsutum, H. vulgare, Sorghum sp., V. radiata,  S. cerevisiae,  D. melanogaster

not analyzed 

no confirmed exceptions from predicted reactivity known in the moment

antibody in concentration 1 µg/ml was sufficient for detection of HDEL-containing proteins in 10 µg of yeast cell lysate by colorimetric western blot. Clone 2E7.
For western blot results,  immunofluorescence and immunogold images please referr to Napier et al. 1992

Luo et al.  (2006). GRP78/BiP is required for cell proliferation and protecting the inner cell mass from apoptosis during early mouse embryonic development. Mol Cell Biol. 26(15): 5688-5697. Napier et al. (1992). Immunological evidence that plants use both HDEL and KDEL for targeting proteins to the endoplasmic reticulum. J Cell Sci. 102: 261-271.