Plant/Algal cell antibodies / Membrane transport system / Vacuolar membrane

product information

V-ATPase is an enzyme responsible for acidification of intracellular compartments. Subunit H is part of the peripheral V1 complex. Alternative name:  vacuolar proton pump subunit H, vacuolar H(+)-ATPase subunit H

KLH-conjugated synthetic peptide conserved in Arabidopsis thaliana V-ATPase subunit H Q9LX65

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

ELISA (ELISA), western blot (WB)

collection of antibodies to vacuolar proteins

AS09 466 | anti-V-ATPase subunit a | vacuolar H+ ATPase, subunit a

AS09 467 | anti-V-ATPase subunit A | vacuolar H+-ATPase

AS09 503 | anti-V-ATPase, B | vacuolar ATP synthase subunit beta

AS09 468 | anti-V-ATPase subunit c | vacuolar H+-ATPase, subunit c (16 kDa)

ASS09 497 | anti- V-ATPase subunit D |V-type proton ATPase subunit D

AS07 213 | anti-V-ATPase subunit E of tonoplast H+ATPase

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few  µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Protocol of isolation of vacuolar membranes can be found here.

application information

1: 8000 (ELISA), 1: 2000 with standard ECL (WB)

50  kDa

Arabidopsis thaliana

dicots including: Spinacia oleracea, monocots including: Hordeum vulgare, Oryza sativa, trees: Picea sitchensis, Populus balsamifera subsp. trichocarpa, algae: Chlamydomonas reinhardtii

no confirmed exceptions from predicted reactivity known in the moment

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it  contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Manufactured by Operon Biotechnologies.

to be added when available