Plant/Algal cell antibodies / Membrane transport system / Vacuolar membrane

product information

Nitrate transporter functions in transporter activity in response to jasmonic acid stimulus and response to wounding. This protein is coded by ntp2 gene.

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana nitrate transporter Q9SZY4

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

ELISA (ELISA), western blot (WB)

collection of antibodies to other proteins localized in tonoplast

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

This protein is present in plant tissues in very low levels.

Protocol for isolation of plant vacuolar membranes can be found here.

application information

1: 8000 (ELISA), 1: 1000 with standard ECL (WB)

63.5 | 70 kDa (Arabidopsis thaliana)

Arabidopsis thaliana, Raphanus sativus

Arabidopsis thaliana, Raphanus sativus

no confirmed exceptions from predicted reactivity known in the moment

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it  contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Manufactured by Operon Biotechnologies.

to be added when available

application example

5 µg and 10 µg of vacuolar membrane fraction/lane from Raphanus sativus  were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-AJ011604 | nitrate transporter antibodies (AS09 473, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.