Plant/Algal cell antibodies / Membrane transport system / Vacuolar membrane

product information

TIPs belong to MIP/aquaporin protein family. Alternative name of radish TIP2;1 (delata) is delta-VM23.

KLH-synthetic peptide conserved in Raphanus sativus TIP2;1 (delta) O82142

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

ELISA (ELISA), western blot (WB)

collection of antibodies to tonoplast proteins

AS09 492 | anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma)

AS09 493 | anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma)

AS09 510 | anti-TIP2;1 | tonoplast intrinistic protein 2-1

AS09 511 | anti-TIP2;2 | tonoplast intrinistic protein 2-2 (C-terminal)

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few  µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

42 kDa dimer is also detected by those antibodies.

Protocol for isolation of plant vacuolar membranes can be found here.

application information

1: 8000 (ELISA), 1: 1000 with standard ECL (WB)

24.9 | 23 kDa

Raphanus sativus

dicots

no confirmed exceptions from predicted reactivity known in the moment

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it  contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Manufactured by Operon Biotechnologies.

to be added when available 

application example

crude membrane of yeast cells expressing recombinaTIP2;1 (RsTIP2;1) and TIP1;1 (RsTIP1;1) proteins were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-TIP2;1 antibodies (AS09 494, 1:2000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.
Left panel: antibody incubacted without peptide, right panel with peptide used to elicit this antibody (peptide competition assay)