Cat | Catalase

378 €

AS09 501 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, B. napus, Ho. vulgare, N. bentamina, O.sativa, Z.mays

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background

Catalase is an enzyme found in most living organisms which is catalazying decomposition of hydrogen peroxide to water and oxygen. In plant cells catalase is found in peroxisomes. This enzyme is involved in photorespiration and symbiotic nitrogen fixation.

immunogen

KLH-conjugated peptide chosen from know plant catalase sequences including Arabidopsis thaliana isoforms: catalase-1 (Q96528, At1g20630), catalase-2 (P25819, At4g35090), catalase-3 (Q42547, At1g20620);

antibody format

rabbit

polyclonal,

serum

lyophilized

quantity

200 µl

storage

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

western blot (WB)

related products

AS08 374 | anti-KatG | catalase peroxidase (HPI), cyanobacterial

additional information

This antibody is recognizing all three isoforms of Arabidopsis thaliana catalase.

application information
recommended dilution		1: 1000 with standard ECL (WB)
expected \| apparent MW		57 \| 55 kDa
confirmed reactivity		Arabidopsis thaliana, Brassica oleracea, Hordeum vulgare, Nicotiana bentamina, Oryza sativa, Solanum lycopersicum, Spinacia oleracea, Zea mays
predicted reactivity		dicots including: Gossypium mexicanum, Glycine max, Vitis vinifera, trees: Populus jackii
not reactive in		no confirmed exceptions from predicted reactivity known in the moment
additional information		To obtain reactivity with Solanum lycopersicum urea gel needs to be apply. Please, contact us for more details.
selected references		Marok et al. (2013). A drought-sensitive barley variety displays oxidative stress and strongly increased contents in low-molecular weight antioxidant compounds during water deficit compared to a tolerant variety. J. Plant Physioology, Feb 8. Ray et al. (2011). Expression and purification of soluble bio-active rice plant catalase-A from recombinant Escherichia coli. J. Biotechnology

western blot using anti plant catalase antibody

10 µg of total protein from Arabidopsis thaliana Col0 (1), Cat2-(Col0) (2), Ler0 (3), Cat2-(Ler0) (4), Zea mays (5), Oryza sativa (6), Brassica oleracea (7), Nicotiana bentamina (8) were extracted with 60mM Tris pH 6.9, 10mM DTT, 20% glycerol, 1mm PMSF were separated on 12.5% SDS-PAGE and blotted 1h to PVDF. Blot was blocked with 3% skim milk in PBS+0.05% Tween20 for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation in the same buffer. The antibody solution was decanted and the blot was rinsed briefly three times, then washed 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, Agrisera, AS09 602) diluted to 1:50 000 in 3% skim milk in PBS+0.05% Tween20 for 1h at RT with agitation. The blot was washed as above and developed for 1 min with Western Lightning Plus-ECL ( PerkinElmer )according to the manufacturers instructions. Exposure time was 5min. in ChemiDoc XRS+ (Biorad ).

Courtesy of Brigitte van de Cotte, Gent University, Belgium

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