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Plant/Algal cell antibodies / Nitrogen metabolism


Urease, Affinity purified

Art no: AS09 561
Price: 360
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product information

background  

Urease (EC=3.5.1.5) is an enzyme involved in nitrogen metabolism. It is catalyzes the hydrolysis of urea into carbon dioxide and ammonia.

immunogen  

urease isolated and purified from Canavalia ensiformis (common jack-beans)

antibody format  

rabbit

polyclonal

affinity purified IgG in phosphate buffer saline,

lyophilized

quantity  

1 mg

for reconstitution please add 0.5 ml of sterile destilled water.

storage  

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications  

western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)

related products  

AS09 559 | anti-urease

AS09 560 | anti-urease (biotinylated)

additional information  

antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

application information

recommended dilution  

1: 100 - 1: 20 000 for techniques listed above

expected | apparent MW  

91 kDa

confirmed reactivity  

Canavalia ensiformis (common jack-bean)

predicted reactivity  

dicots including: Arabidopsis thaliana, Glycine max, Ricinus communis

not reactive in  

no confirmed exceptions from predicted reactivity known in the moment

additional information  

Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran.

selected references  

to be added when available



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