Urease, Affinity purified

378 €

AS09 561 | clonality: polyclonal | host: rabbit | reactivity: Canavalia ensiformis (common jack-bean)

product information

background

Urease (EC=3.5.1.5) is an enzyme involved in nitrogen metabolism. It is catalyzes the hydrolysis of urea into carbon dioxide and ammonia.

immunogen

urease isolated and purified from Canavalia ensiformis (common jack-beans)

antibody format

rabbit

polyclonal

affinity purified IgG in phosphate buffer saline,

lyophilized

quantity

1 mg	for reconstitution please add 0.5 ml of sterile destilled water.

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)

related products

AS09 559 | anti-urease

AS09 560 | anti-urease (biotinylated)

additional information

antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

application information
recommended dilution		1: 100 - 1: 20 000 for techniques listed above
expected \| apparent MW		91 kDa
confirmed reactivity		Canavalia ensiformis (common jack-bean)
predicted reactivity		dicots including: Arabidopsis thaliana, Glycine max, Ricinus communis
not reactive in		no confirmed exceptions from predicted reactivity known in the moment
additional information		Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran.
selected references		to be added when available

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