Plant/Algal cell antibodies / Oryza sativa

background | this kit is intended to use during teaching labs and contains following antibodies and protein standards:

BOX 1 PRIMARY antibodies:
Anti-RbcL (Rubisco Large Subunit) AS03 037-10
Anti-PsbA (D1 protein, Photosystem II) AS05 084-10
Anti-PsaC (Photosystem I) AS10 939-10
Anti-AtpB AS05 085-10
Anti-Lhcb1 AS01 004-10

quantity |10 ul aliquot of each antibody, which will allow at least 10 Western Blot detections

Final dilution of primary antibodies can differ depending upon plant material used.

BOX 2 Protein standards:
Rubisco AS01 017S
PsbA (Photosystem II) AS01 016S
PsaC (Photosystem I) AS04 042S
AtpB AS03 030S

quantity |100 µl aliquot of each protein standard

Suggested experiments as well as respective protocols will be included.
Educational Kit - Photosynthesis has been tested by:
Umeå Plant Science Centre, Sweden
During a Plant Physiology course at the University of Tennessee, USA.
If you would like to contact directly person responsible for the course and testing of antibodies - please contact us.

Below are comments of students who attended the labs with Educational Kit:
"I think that the process of identifying the proteins using the antibodies was the coolest. It was neat to do something that is working even though you can’t see it. The whole binding process of antibodies is unique."

"I enjoyed the portion of the experiment involving the antibody/antigen reaction and the immunodetection. This type of detection gives the researcher direct proof of the presence of specific types of protein present within the samples. I have performed similar detections unvolving ELISA and this was my first time to use X-ray film. We previously used absorbance values to indicate the presence and amount of proteins present."

"I thought it was a very good idea that we got to pick out which samples to use on our gels. This made it more interesting for me because I was researching something that I chose. The X-ray developing portion is also really interesting because it is something that I probably wouldn’t have gotten to do had we not done it in this lab."

"The most interesting parts of the experiments were physically loading the dyes and processing the gels. There techniques will likely prove useful to my future."

" I enjoyed this course and I particularly liked experiments 15 & 16 which dealt with protein electrophoresis and immunodetection. I had never used X-ray film."

"I really enjoyed the western blot lab. The electrophoresis part was not as neat because I was familiar with this process, but getting to do the western and the immunodetection was really awesome because it was new and the results were pretty good."

"The electrophoresis was really great. Keep this experiment; it is a good one - very informative lab. I liked the aspect of having to create hypotheses about the expected results before completing the experiment."