Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.
immunogen
ascorbate oxidase purified from Cucurbita sp.
antibody format
rabbit
polyclonal
total IgG in PBS pH 7.4
lyophilized
quantity
100 µl
for reconstitution add 100 µl of sterile water.
storage
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
dicots including Glycine max, monocots including Oryza sativa
not reactive in
no confirmed exceptions from predicted reactivity known in the moment
additional information
antibody can be used as a marker of apoplast
selected references
Kielbowicz et al. (2008) The organ-dependent abundance of a Solanum lipid transfer protein is up-regulated upon osmotic constraints and associated with cold acclimation ability. J. Expo Bot. 59:2191-2203.
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