Plant/Algal cell biology / Oryza sativa

product information

Pairing and synapsis of homologous chromosomes is required for normal chromosome segregation and the exchange of genetic material via recombination during meiosis. Synapsis is complete at pachytene following the formation of a tri-partite proteinaceous structure known as the synaptonemal complex (SC). In yeast, HOP1 is essential for formation of the SC, and localises along chromosome axes during prophase I. Homologues in Arabidopsis (AtASY1), Brassica (BoASY1) and rice (OsPAIR2) have been isolated through analysis of mutants that display decreased fertility due to severely reduced synapsis of homologous chromosomes. Analysis of these genes has indicated that they play a similar role to HOP1 in pairing and formation of the SC through localisation to axial/lateral elements of the SC.

recombinant ASY1 protein produced in E.coli prepared from the sequence of Triticum aestivum ASY1 A7TVU8

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB), ELISA (ELISA)

AS08 338 | anti-ASY1 mouse antibody

to be added when available

application information

1: 1000 with standard ECL (WB) , 1: 10 000 (ELISA)

66.3

Triticum aestivum

Oryza sativa

no confirmed exceptions from predicted reactivity known in the moment

to be added when available

Boden et al. (2007). Expression and functional analysis of TaASY1 during meiosis of bread wheat (Triticum aestivum).BMC Mol. Biol. 8:65