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product information
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| background |
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Multi-subunit complex of cytb6/f is a crucial component for the photosynthetic electron transport chain of higher plants, green algae and cyanobacteria. This complex is catalyzing oxidation of quinols and the reduction the reduction of plastocyanin. This reaction allows to establish the proton force required for the ATP synthesis. Four major subunits build the complex: the petA gene product corresponding to a c-type cytochrome (cytf), the petB gene product corresponding to a b-type/c’-type cytochrome with three haems (cyt b6), the petD gene product (subunit IV, or suIV), and the petC gene product, corresponding to the Rieske/Iron/sulfur protein. |
| immunogen |
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native Zea mays cytb6 protein run on SDS PAGE and excised from a gel piece |
| antibody format |
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rabbit |
polyclonal |
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serum |
lyophilized |
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| quantity |
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100 µl |
for reconstitution add 100 µl of sterile water. |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB), immunogold (IG), immunoprecipiation (IP) |
| related products |
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AS03 034 | anti-Cyt b6 | thylakoid membrane cytochrome b6 protein, N terminal collection of antibodies to proteins involved in photosynthetic electron transfer |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1 : 5000 with alkaline phosphatase (WB), 1: 20 (IG), 1:1000 (IP) |
| expected | apparent MW |
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23-25 kDa |
| confirmed reactivity |
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Arabidopsis thalian, Zea mays |
| predicted reactivity |
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di and monocots |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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immunogold localization has been done in leaf material of Arabidopsis thaliana |
| selected references |
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to be added when available |
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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