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product information
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| background |
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Multi-subunit complex of cytb6/f is a crucial component for the photosynthetic electron transport chain of higher plants, green algae and cyanobacteria. This complex is catalyzing oxidation of quinols and the reduction the reduction of plastocyanin. This reaction allows to establish the proton force required for the ATP synthesis. Four major subunits build the complex: the petA gene product corresponding to a c-type cytochrome (cytf), the petB gene product corresponding to a b-type/c’-type cytochrome with three haems (cyt b6), the petD gene product (subunit IV, or suIV), and the petC gene product, corresponding to the Rieske/Iron/sulfur protein. |
| immunogen |
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purified, native cytochrome f from maize excised from SDS PAGE gel |
| antibody format |
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rabbit |
polyclonal |
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serum |
lyophilized |
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| quantity |
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100 µl |
for reconstitution add 100 µl of sterile water. |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB), immunoprecipitation (IP) |
| related products |
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antibodies to proteins involved in electron transfer |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1 : 5000 with alkaline phosphatase (WB), 1: 500 (IP) |
| expected | apparent MW |
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31-32 kDa |
| confirmed reactivity |
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Zea mays |
| predicted reactivity |
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monocts, algae |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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Antibody will react with mature and pre-protein and can be used for immunoprecipiation of chloroplast encoded proteins. |
| selected references |
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not available at the moment |
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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