Plant/Algal cell antibodies / Oryza sativa

product information

L13 protein is localized in the cytoplasm. It belongs to the ribosomal protein L13e family. Alternative name: protein BBC1 homolog

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana  P41127

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

ELISA (ELISA), western blot (WB)

AS09 477 | anti-S14 | 40S ribosomal protein S14-1

AS08 309 | anti-S1 | 30S ribosomal protein S1

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few  µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

application information

1: 8000 (ELISA), 1: 1000 with standard ECL (WB)

23.7 | 29 kDa (Arabidopsis thaliana)

Arabidopsis thaliana

dicots including: Brassica napus, Nicotiana tabacum, Ricinus communis, Vitis vinifera, monocots including: Oryza sativa, Zea mays,  trees: Picea excelsa, Populus balsamifera, algae: Chlamydomonas reinhardii,

diatoms

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it  contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Manufactured by Operon Biotechnologies.

to be added when available

application example

1 µg and 10 µg of crude membrane fraction/lane from Arabidopsis thaliana  were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-L13 antibodies (AS09 478, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.