Plant/Algal cell antibodies / Oryza sativa

product information

PIP1;3 is a plasma membrane aquaporine which facilitates trasport of water across cell membrane. Alternative names: aquaporin PIP 1-3, water channel protein RWC3, RWC-3

peptide derived from Oryza sativa PIP1-3 Q9SXF8

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

ELISA (ELISA), western blot (WB)

collection of antibodies to plasma membrane proteins

AS09 505 | anti-PIP1s | aquaporin

AS09 506 | anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1

AS09 507 | anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1

AS09 508 | anti-PIP2;5 | aquaporin, plasma membrane intrinistic protein 2-5

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few  µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Protocol for isolation of plasma membrane can be found here.

application information

1: 8000 (ELISA), 1: 2000 with standard ECL (WB)

30.7 |  26-29 kDa (Oryza sativa)

Oryza sativa

monocots including: Hordeum vulgare, Stipa sp. Triticum aestivum,

no confirmed exceptions from predicted reactivity known in the moment

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it  contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Manufactured by Operon Biotechnologies.

Sakurai et al. (2008). Tissue and cell-specific localization of rice aquaporins and their water transport activities- Plant Cell Physiol. 49: 30-39. 

application example

1.5 µg of crude membrane fraction/lane from Oryza sativa L. Akitakomachi  were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-PIPs1;3 antibodies (AS09 504, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

Below: Immunospecificity of anti.PIP1;3 antibodies to recombinant PIP proteins expressed in yeast cells. 1.5 µg protein/lane.

Most aquaporins show the dimerc forms in addition to monomer even in the presence of SDS.