Plant/Algal cell antibodies / Oryza sativa

product information

PIPs proteins are aquaporins which facilitate the transport of water and small neutral molecules across cell membrane.

KLH-conjugated synthetic peptide derived from Raphanus sativus PAQ2 O80369

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

ELISA (ELISA), western blot (WB)

collection of antibodies to plasma membrane proteins

AS09 489 | anti-PIP1;1, PIP1;2, PIP1;3 | aquaporins

AS09 490 | anti-PIP2;2 | plasma membrane aquaporin 2b

AS09 491 | anti-PIP2;1,PIP2;2,PIP2;3 |plasma membrane intrinistic protein 2-1,2-2, 2-3

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few  µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Protocol for plasma membrane preparation can be found here.  

application information

1: 8000 (ELISA), 1: 1000 with standard ECL (WB)

30.34 | 28  kDa (Raphanus sativus)

Arabidopsis thaliana, Oryza sativa, Raphanus sativus

dicots including: Brassica napus, Gossypium mexicanum, 

no confirmed exceptions from predicted reactivity known in the moment

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it  contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Peptide used to elicit this antibody is also conserved in Arabidopsis thaliana PIP2-1,PIP2-2, PIP2-3

Manufactured by Operon Biotechnology

Markus G. et al., (2004). Arabidopsis Immunophilin-like TWD1 Functionally Interacts with Vacuolar ABC Transporters.Mol. Biol. Cell. 15, 3393-3405.

application example

1 µg and 10 µg of crude membrane fraction/lane from Arabidopsis thaliana  were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-PIP2;1 antibodies (AS09 488, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.