Plant/Algal cell antibodies / Oryza sativa

product information

PRK ribulose-5-P-kinase| phosphoribulokinase is an enzyme that catalyzes the chemical reaction ATP + D-ribulose 5-phosphate to  ADP + D-ribulose 1,5-bisphosphate

KLH-conjugated synthetic peptide conserved known PRK sequences including Zea mays B8A064 PRK

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

collection of antibodies to various photosynthetic proteins

to be added when available

application information

1. 1000 (WB)

44 | 39 kDa (Zea mays)

Hordeum vulgare, Zea mays

Chlamydomonas reinhardtii, Physcomitrella patens, cyanobacteria

no confirmed exceptions from predicted reactivity known in the moment

to be added when available

to be added when available

application example

10 µg of total leaf protein extracted with PEB (AS08 300) from (1) Zea mays and (2) Hordeum vulgare were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 80 min (30V) to nitrocellulose. Filter was blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-PRK (AS09 464, 1:1000, 1h) and secondary anti-rabbit (1:40000, 1h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with SuperSignal West Pico (Thermo Scientific) using a GenoPlex Chemi CCD (accumulated signal 10 x 30s exposure, bin 2x2).