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product information
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| background |
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PSII reaction centre components are generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC. |
| immunogen |
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recombinant sorghum OE23 protein |
| antibody format |
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rabbit; |
polyclonal; |
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serum; |
lyophilized |
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| quantity |
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100 µl |
- for reconstitution add 100 µl of sterile water |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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Western blot (WB) |
| related products |
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AS05 092 | anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII AS06 142-33 | anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII AS06 167 | anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII AS06 142-16 | anti-PsbQ | 16 kDa protein of the oxygen evolving complex (OEC) of PSII |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1: 5 000 with standard ECL (WB) |
| expected | apparent MW |
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23 kDa |
| confirmed reactivity |
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Arabidopsis thaliana, Sorghum bicolor, Zea mays |
| predicted reactivity |
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monocot including: Hordeum vulgare, Oryza sativa |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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to be added when available |
| selected references |
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to be added when available |
application example | 5 µg of total protein from (1) Arabidopsis thaliana total leaf extract; (2) Zea mays total leaf extract were separated in a 12% or 5-15% gradient gel following by transfer to nitrocellulose membrane. Membrane was blocked with TBST + 4% non-fat dried milk, 20 min following by three washes in TBST. Incubation time with primary and secondary antibodies was 1 hr primary, 30 min for secondary antibodies. Manufacturer of secondary antibodies: Bio-Rad | |
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use LiveChat option in the left menu or contact us at support@agrisera.com
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