Plant/Algal cell antibodies / Oryza sativa

product information

Rubisco catalyzes the rate-limiting step of CO2 fixation in photosynthesis.  This enzyme contains two subunits, each present in eight copies. In plants and green algae, 55-kD large subunit is coded by the chloroplast rbcL gene, and the 15-kD small subunit is coded by a family of nuclear RbcS genes.

purified, native Rubisco small subunit of Pisum sativum P07689

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS07 259 | anti-RbcS | Rubisco small subunit  (SSU)

AS03 037 | anti-RbcL rabbit antibody

AS07 218 | Rubisco | 557 kDa hexadecamer

AS09 409 | Rubisco quantitation kit

application information

1 : 10 000 with standard ECL (WB)

20 | 15 kDa

Arabidopsis thaliana, Hordeum vulgare, Oryza sativa, Pisum sativum ,Zea mays

 dicots including: Glycine max, Nicotiana tabacum, Solanum lycopersicum

cyanobacteria

this antibody will also detect an in vitro translated precursor prSSU but not the transit peptide

Law et al. (2012). Nucleotide and RNA metabolism prime translational initiation in the earliest events of mitochondrial biogenesis during Arabidopsis thaliana germination. Plant Physiol. February 2012.

Krupinska et al. (2011). An alternative strategy of dismantling of the chloroplasts during leaf senescence observed in a high yield variety of barley. Plant Physiol. 19