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product information
|
| background |
|
Sar1 belongs to a small GTPase superfamily and GTP binging activity. This protein is involved in intracellular protein transport. There are two different non-clathrin-coated vesicles that are responsible for transport between ER and the Golgi. Coat protein complexes involving membrane-associated GTP binging proteins – Arf1 and Sar1p for COPI and COPII are needed for formation of COP-coated vesicles. Sar1p is a cytosolic protein. It is temporarily recruited onto the membranes of the ER. |
| immunogen |
|
GST fusion of full length recombinant Sar1 of Arabidopsis thaliana Q8VYP7 |
| antibody format |
|
rabbit |
polyclonal |
|
serum |
lyophilized |
|
| quantity |
|
200 µl |
for reconstitution add 200 µl of sterile water. |
|
| storage |
|
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
|
western blot (WB), immunogold (IG) |
| related products |
|
AS08 327 | anti-Sec21p | gamma subunit, COP vesicles, Golgi marker, rabbit antibody AS08 325 | anti-Arf1 | ADP-ribosylation factor 1 rabbit antibody |
| additional information |
|
For immunogold experiments plant tissue has been fixed with GA in PFA/PIPES. LR White resin has been used. Tested species were: Triticum aestivum, Panicum miliaceum, Panicum maximum, Echinochloa crus-galli Eragrostis neomexicana, Digitaria sanguinalis. Publication in preparation. |
application information
|
| recommended dilution |
|
1: 500 with standard ECL (WB), 1: 50 (IG) |
| expected | apparent MW |
|
21 kDa (Arabidopsis thaliana) |
| confirmed reactivity |
|
Arabidopsis thaliana, Echinochloa crus-galli Eragrostis neomexicana, Digitaria sanguinalis, Pteris vittata (fern), Panicum miliaceum, Panicum maximum, Triticum aestivum, Zea mays
|
| predicted reactivity |
|
dicots including Brassica campestris, Solanum tuberosum monocots including Triticum aestivum, Oryza sativa, tree: Populus trichocarpa, moss Physcomitrella patens |
| not reactive in |
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Nicotiana tabacum |
| additional information |
|
to be added when available |
| selected references |
|
Tnani et al. (2011). Protein composition analysis of oil bodies from maize embryos during germination. Plant Physiol. 168 (5): 510-513. |
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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