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product information
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| background |
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STN7 (serine/threonine protein kinase) (EC=2.7.11.1) in Arabidopsis thaliana, and its Stt7 homologue in Chlamydomonas reinhardtii, is required for LHCII phosphorylation and for state transitions. The loss of this thylakoid-associated kinase blocked stn7 (stt7)-deficient mutants in state 1. Alternative names: Protein STATE TRANSITION 7, Stt7 homolog.
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| immunogen |
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KLH-conjugated synthetic peptide (amino acids 128 – 141) specific for Arabidopsis thaliana STN7 serine/threonine kinase At1g68830 |
| antibody format |
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| rabbit |
polyclonal |
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affinity purified serum |
lyophilized |
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| quantity |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB) |
| related products |
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AS10 1601 | STN8 kinase | serine/threonine-protein kinase STN8, chloroplastic |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1: 2000 with standard ECL (WB) |
| expected | apparent MW |
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63.2 | 44 kDa (on urea gel), 55 kDa (no urea)
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| confirmed reactivity |
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Arabidopsis thaliana, Nicotiana tabacum |
| predicted reactivity |
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dicots including: Ricinus communis, Vitis vinifera, monocts including: Oryza sativa, Zea mays
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| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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Stn7 is a nuclear encoded protein which is localized in chloroplast, hence posses a chloroplastic target peptides (cTP) at the beginning of the amino acid sequence which is cut off. Accroding to TargetP (program that predicts the length of the cTP:s) the length of cTP for Stn7 is 45aa. STN7 portein mobility can be also affected by urea present in the gel.
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| selected references |
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to be added when available, antibody released in Febraury 2012
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application example 
Thylakoids 3 ug of chlorophyll/lane from Arabidopsis thaliana were extracted according to Sirpiö et al (2011, Methods Mol Biol. 2011;775:19-30) and were separated on 12 % SDS-PAGE containing 6 M urea and blotted 1h to PVDF. Blots were blocked with 5% milk in TTBS for 1h at room temperature (RT) with slow agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 over night at 4°C with slow agitation in 1 % milk TTBS . The antibody solution was decanted and the blot was rinsed briefly only once, then washed once for 15 min only in TBS-T at RT with vigorous agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated ) diluted to 1:10 000 in 1% milk in TTBS for 2h at 4°C with slow agitation. The blot was washed as above and incubated for 5 min with ECL-solution according to the manufacturers instructions. Exposure time was 1 min. Courtesy Dr. Maija Holmström, University of Turku, Finland
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