|
product information
|
| background |
|
Multi-subunit complex of cytb6/f is a crucial component for the photosynthetic electron transport chain of higher plants, green algae and cyanobacteria. This complex is catalyzing oxidation of quinols and the reduction the reduction of plastocyanin. This reaction allows to establish the proton force required for the ATP synthesis. Four major subunits build the complex: the petA gene product corresponding to a c-type cytochrome (cytf), the petB gene product corresponding to a b-type/c’-type cytochrome with three haems (cyt b6), the petD gene product (subunit IV, or suIV), and the petC gene product, corresponding to the Rieske/Iron/sulfur protein. |
| immunogen |
|
KLH-conjugated peptide chosen from N terminal sequence of cytb6 conserved in Arabidopsis thaliana P56773 and Chlamydomonas reinhardtii NP_958365.1 |
| antibody format |
|
rabbit |
polyclonal |
|
serum, |
lyophilized |
|
| quantity |
|
100 µl |
for reconstitution add 100 µl of sterile water. |
|
| storage |
|
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
|
western blot (WB) |
| related products |
|
AS08 330 | anti-PetC rabbit antibody AS06 202 | anti-cytochrome c6 Collection of antibodies to Arabidopsis proteins Collection of antibodies to Chlamydomonas proteins |
| additional information |
|
to be added when available |
application information
|
| recommended dilution |
|
1: 10 000 with standard ECL (WB) |
| expected | apparent MW |
|
23-25 kDa |
| confirmed reactivity |
|
Arabidopsis thaliana, Chlamydomonas reinhardtii, Thalassiosira weisflogii and Thalassiosira pseudonana |
| predicted reactivity |
|
dicots including Pisum sativum, Spinacia oleracea and monocots including Oryza sativa, Hordeum vulgare, deciduous flowering plants Populus alba |
| not reactive in |
|
Synechocystis sp. 6803, Heterosigma akashiwo |
| additional information |
|
n.a. |
| selected references |
|
n.a. |
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
Save as PDF
|