Plant/Algal cell antibodies / Photosynthesis / Electron transfer

product information

Ferredoxins are acidic, low molecular weight, soluble iron-sulfur proteins found in various organisms. Iron-sulfur proteins are defined as proteins carrying iron-sulfur cluster(s) in which the iron is at least partially coordinated by sulfur. The protein acts as multifunctional electron carriers in diverse redox systems. The chloroplast ferredoxin is involved in both cyclic and non-cyclic photophosphorylation reactions of photosynthesis and other reductive reactions in the chloroplast.

native ferredoxin purified from Spinacia oleracea

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

collection of antibodies to other proteins involved in electron transfer

In Arabidopsis thaliana leaf extracts there is a strong cross-reactivity at 20 kDa

application information

1: 1000 with alkaline phosphatase or standard ECL (WB)

10 (Spinacia oleracea); 16.7 (Arabidopsis thaliana), 13.7 (Chlamydomonas reinhardtii)

Arabidopsis thaliana, Spinacia oleracea, Chlamydomonas reinhardtii, Hordeum vulgare

dicotyl plants

no confirmed exceptions from predicted reactivity known in the moment

load per well: 20-40  µg/lane required for Arabidopsis thaliana, 2-10 µg for other species

application example

20 or 40 µg of total protein from Arabidopsis thaliana (WT)
leafs or ferredoxin mutant fd2 were separated on 15 %
acrylamide gel with 6 M urea. Filters were blotted on PVDF,
blocked (1 h) with 5 % milk, incubated with 1: 1000 anti-ferredoxin (over night in 4ºC) in 1 % milk/TBS-T) followed by incubation with 1: 10000 secondary antibody (2 h) coupled to HRP and visualization with standard ECL.