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product information
|
| background |
|
HemH is ptotoporphyrin ferrochelatase of heme biosynthesis. |
| immunogen |
|
KLH-conjugated peptide derived from cyanobacterial ferrochelatase protein sequence |
| antibody format |
|
rabbit |
polyclonal |
|
serum |
lyophilized |
|
| quantity |
|
100 µl |
for reconstitution add 100 µl of sterile water |
|
| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
|
western blot (WB) |
| related products |
|
AS07 228 | HemH | protoporphyrin ferrochelatase (reacts with higher plants) |
| additional information |
|
to be added when available |
application information
|
| recommended dilution |
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1: 3000 with standard ECL, West pico (WB) |
| expected | apparent MW |
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46 kDa |
| confirmed reactivity |
|
Synechocystis sp. PCC 6803 |
| predicted reactivity |
|
cyanobacteria |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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loads higher than 0.5 µg of chlorophyll per well are not recommended |
| selected references |
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to be added when available |
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application example
Membrane fraction (0.5 µg chl), prepared from Synechocystis sp. PCC 6803, was separated on 12% SDS-PAGE and blotted in 2 hours at RT to PVDF membrane. Membrane was blocked for 1h using 0.2% tween 20 in TBS, probed with anti-PsbI (1:10 000) antibody in TTBS overnight at 8ºC and secondary anti-rabbit in TTBS (1:10000,1h, room temperature). Blot was developed for 5 min in West pico substrate and image of the blot was obtained using Fuji LAS 4000. Courtesy Dr. Roman Sobotka, Laboratory of Photosynthesis, Trebon,
Czech Republic
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||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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