Plant/Algal cell antibodies / Photosynthesis / Chlorophyll

product information

Chlorophylls are one of the most abundant classes of natural pigments and have an essential role in radiant energy absorptions during photosynthesis in bacteria, algae, and higher plants. Chlorophylls occur as noncovalently bound components of pigment-protein complexes, chloroplast-localized light-harvesting antennas LHC and photosynthetic reaction centers of PSI and PSII. Upon illumination the arrest in chlorophyll biosynthesis is ended and the etiolated parts of the plant and enzymatic photoreduction of protochlorophyllide (Pchlide) to chlorophyllide (Chlide), catalysed by POR enzyme.

native wheat POR protein isolated from a gel piece

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS05 067 | POR | protochlorophilide oxidoreductase (200ul)

AS08 300 | protein extraction buffer for plant and algal samples

to be added when available

application information

1:2 000 with standard ECL (WB)

36-37 kDa (Arabidopsis thaliana)

Arabidopsis thaliana, Horderum vulgare, Pisum sativum, Triticum aestivum and cyanobacteria

n.a.

no confirmed exceptions from predicted reactivity known in the moment

to be added when available

Peter et al. (2010). Mg protoporphyrin monomethylester cyclase deficiency and effects on tetrapyrrole metabolism in different light conditions. Plant Cell Physiol. 51(7):1229-1241.

Wei et al (2008). Heterotrimeric G-protein is involved in phytochrome A-mediated cell death of Arabidopsis hypocotyls. Cell Research 18:949-960.