This product includes following antibodies: AS01 004 Lhcb1 | LHCII type I chlorophyll a/b-binding protein AS01 003 Lhcb2 | LHCII type II chlorophyll a/b-binding protein AS06 117 Lhcb4 | CP29 (Lhcb4) homolog, Chlamydomonas AS09 407 Lhcb5 | CP26 (Lhcb5) homolog, Chlamydomonas AS09 408 Lhcbm5 | chlorophyll a-b binding protein of LHCII |
product information
|
| background |
|
The outer light-harvesting antenna of the photosystems (PSI and PSII) of the green unicellular alga Chlamydomonas reinhardtii is composed of pigment-binding proteins belonging to the Lhc family highly conserved in photosynthetic eukaryotes. Some of the Lhc-subtypes of Chlamydomonas share sufficient high similarity with the respective functional homologs in plants to allow specific detection with antisera generated against conserved peptide domains from plant Lhc-proteins. |
| immunogen |
|
Lhcb1 - BSA-conjugated peptide Lhcb2 - BSA-conjugated peptide Lhcb4 - KLH-conjugated synthetic peptide derived from Lhcb4 (CP29, Lhcbm4) protein sequence from Chlamydomonas reinhardtii (Q93WD2) Lhcb5 - KLH-conjugated synthetic peptide derived from Chlamydomonas reinhardtii Lhcb5 protein sequence (Q9FEK6) Lhcbm5 - SDS-PAGE purified polypeptide from Chlamydomonas reinhardtii LHCII-type II-enriched fractions |
| antibody format |
|
rabbit |
polyclonal |
|
total IgG in PBS pH 7.4 (Lhcb1 and Lhcb2), serum (Lhcb4, Lhcb5, Lhcbm5) |
lyophilized |
|
| quantity |
|
100 µl (Lhcb4, Lhcb5, Lhcbm5) 200 µl (Lhcb1, Lhcb2) |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
|
western blot (WB) |
| related products |
|
other antibodies against pigment-binding proteins LHC |
| additional information |
|
to be added when available |
application information
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| recommended dilution |
|
1:2000 with standard ECL (WB) (Lhcb1) 1:5000 with standard ECL (WB) (Lhcb2,Lhcb5, Lhcbm5) 1: 10 000 with standard ECL (Lhcb4) |
| expected | apparent MW |
|
25 kDa (Lhcb1, Lhcb2) 29 kDa (Lhcb4) 29 | 30 kDa (Lhcbm5) 30| 26 kDa (Lhcb5) |
| confirmed reactivity |
|
Chlamydomonas reinhardtii |
| predicted reactivity |
|
angiosperms (monocots and dicots), gymnosperms, mosses, green algae (Lhcb1, Lhcb2) |
| not reactive in |
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Lhcb4 - higher plants (use AS04 045 for those organsims), algae, cyanobacteria |
| additional information |
|
to be added when available |
| selected references |
|
Lhcb5 and Lhcbm5 - Takahashi et al. (2006). Identification of the mobile light-harvesting complex II polypeptides for state transitions in Chlamydomonas reinhardtii. PNAS 103:477-482 |
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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