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product information
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| background |
|
B-PE is a phycobiliprotein which is a building block of phycobilisomes, the light-harvesting complex, in red algae and some cyanobacteria. Phycobilisomes are attached to the stromal side of the thylakoid membrane. B-phycoerythrin has both phycoerythrobilin (on alpha and beta subunits) and phycourobilin (on the gamma subunits) chromophores. |
| immunogen |
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native purified B-phycoerythrin of Porphyridium cruentum with attached chromophores |
| antibody format |
|
rabbit |
polyclonal |
|
serum |
lyophilized |
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| quantity |
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200 µl |
for reconstitution add 200 µl of sterile water. |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
|
western blot (WB) |
| related products |
|
AS08 277 | anti-APC | allophycocyanin alpha and beta AS08 278 | anti-R-PC | R-phycocyanin AS08 279 | anti-b-PE | phycoerythrobilin |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1:1000 - 5000 with standard ECL (WB) |
| expected | apparent MW |
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17-20 and 30-34 kDa |
| confirmed reactivity |
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Porphyridium cruentum |
| predicted reactivity |
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red algae, cyanobacteria, cryptomonads |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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to be added when available |
| selected references |
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Gantt & Lipschultz (1974). Phycobilisomes of Porphyridium cruentum: Pigment Analysis. Biochem. 13:2960. Gantt E & C Lipschultz (1977). Probing phycobilisome structure by immuno-electron microscopy. J Phycol. 13:18. |
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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