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product information
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| background |
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HliD (ScpE) protein, high light inducible protein, is LHC-similar stress-induced protein from cyanobacteria. HliD is associated with damaged photosystem II and can serve as a temporary pigment reservoir while photosystem II components are being replaced.
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| immunogen |
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synthetic peptide (amino acids 15 – 30) for HliD protein from Synechocystis sp. PCC 6803. NP_440269.1
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| antibody format |
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| rabbit |
polyclonal |
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serum |
liquid |
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| quantity |
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| storage |
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Short term 4°C. Long term -20°C. Repeated freezing and thawing is not recommended. Solution contains 0.01% sodium azide. |
| tested applications |
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western blot (WB) |
| related products |
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AS10 1615 | nti-HliD | High light inducible protein, larger pack size |
| additional information |
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Using nitrocellulose for western blotting is highly recommended to obtain a signal with this antibody. Pre-immune serum is available to this product upon request. |
application information
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| recommended dilution |
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1: 2000 with standard ECL (WB) |
| expected | apparent MW |
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5 kDa
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| confirmed reactivity |
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Synechocystis sp. PCC 6803 |
| predicted reactivity |
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According to sequence analysis antibody may react with homologous Hli protein(-s) from Anabaena, Thermosynechococcus, Gloeobacter, Prochlorococcus, Synechococcus and Crocosphaera. |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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to be added when available |
| selected references |
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to be added when available |
application example
40 µg of membrane proteins from Synechocystis 6803 WT and DhliD strains was separated on 12-20 % SDS-PAGE and blotted 3h to PVDF. Blot was blocked with 0.2% Tween 20 in TBS for 1h at room temperature with agitation and than incubated in the primary antibody at a dilution of 1: 2 000 overnight at 10°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed twice for 5 min in TBS-T at room temperature with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1: 10 000 in for 1h at room temperature with agitation. Finally, the blot was washed as above, developed for 5 min with ECL (Plus Supersignal West Pico) according to the manufacturers instructions and exposed for 30 min in LAS 4000 imager.
WT and ΔScpE deletion mutants grown at 40 μE m-2s-1 and exposed to HL 2000 μE m-2s-1 for 30 min. Courtesy of Dr. Josef Komenda, Academy of Science of Czech Republic
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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