Plant/Algal cell antibodies / Photosynthesis / LHC

product information

The light-harvesting system of higher plant is composed of pigment-binding proteins belonging to the Lhc multigenic family. Lhc polypeptides are able to bind chlorophyll a, chlorophyll b, and xanthophyll molecules in a suitable structural and dynamic mutual arrangement, ensuring high efficiency of energy transfer processes involved in light-harvesting and photoprotection.

purified LHC complex from Porphyridium cruentum

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS09 407 | anti-Lhcb5 | CP26 (Lhcb5) homolog, Chlamydomonas

AS09 408 | anti-Lhcbm5 | chlorophyll a-b binding protein of LHCII

AS06 117 | anti-Lhcb4 | CP29 (Lhcb4) homolog, Chlamydomonas

to be added when available

application information

1: 1000 with standard ECL (WB)

20-25 kDa

Aureococcus anophagefferens,Guillardia theta,Heterosigma akashiwo, Thalassiosira pseudonana, Porphyridium cruentum

diatoms

no confirmed exceptions from predicted reactivity known in the moment

strongly reactive to 7 Lhc1 light harvesting polypeptides of P. cruentum, reactivity to a few Lhc2 polypeptides of spinach is very low

Tan et al. (1995).Decrease of polypeptides in the PS I antenna complex with increasing growth irradiance in the red alga Porphyridium cruentum. Photosyn. Research 45:1. Wolfe et al. (1994) Evidence for a common origin of chloroplasts with light-harvesting complexes of different pigmentation. Nature 367:566

application example

2 µg of total chlorophyll/lane of total cell extract from (1) Thalassiosira pseudonana, (2) Heterosigma akashiwo , (3) Guillardia theta, (4) Aureococcus anophagefferens, extracted with Agrisera protein extraction buffer PEB, were separated on 13-17% SDS-PAGE and blotted 2h to nitrocellulose. membranes were blocked 1h with 5% low-fat milk powder in PBS-T (0.1% TWEEN 20) and
probed with anti-Lhc1 (AS08 282, 1:6000, 1h) and secondary anti-rabbit (1:5000, 1 h) antibody (HRP conjugated, Bio-rad) in PBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in PBS-T (15, +5, +5, +5 min). All steps were
performed at RT with agitation. Signal was detected with standard ECL. Exposure time was 2 min.

Courtesy of Meriem Alami and Beverley Green.