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product information
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| background |
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The light-harvesting protein Lhca3 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca3 is imported as a precursor from the cytosol into the chloroplast. Upon integration in the thylakoid membrane Lhca3 forms a heterodimer (LHCI-680) with Lhca2 that associates with the PSI core close to PsaF and PsaK. |
| immunogen |
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BSA-conjugated synthetic peptide derived from the Lhca3 protein sequence from Arabidopsis thaliana (At1g61520). This sequence is highly conserved in Lhcb3 proteins from angiosperms (monocots and dicots) and gymnosperms. |
| antibody format |
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rabbit |
polyclonal, |
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total IgG in PBS pH 7.4, |
lyophilized |
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| quantity |
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200 µl |
- for reconstitution add 200 µl of sterile water |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB) |
| related products |
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AS01 005 Lhca1 | PSI type I chloropyll a/b-binding protein AS01 006 Lhca2 | PSI type II chlorophyll a/b-binding protein AS01 008 Lhca4 | PSI type IV chlorophyll a/b-binding protein AS05 082 Lhca5 | PSI type V chlorophyll a/b-binding protein LHC available antibodies against pigment-binding proteins |
| additional information |
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A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065–3073 |
application information
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| recommended dilution |
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1 : 2000 - 1: 5000, detected with standard ECL (WB) |
| expected | apparent MW |
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25 | 25 kDa for Arabidopsis thaliana |
| confirmed reactivity |
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Arabidopsis thaliana, Aracis hypogae, Hordeum vulgare, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Spinacia oleracea, Zea mays |
| predicted reactivity |
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angiosperms (monocots and dicots), gymnosperms |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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to be added when available |
| selected references |
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Yadavalli et al. (2012). Differential degradation of photosystem I subunits under iron deficiency in rice. J Plant Physiol. March 22. Rudowska et al. (2012). Chloroplast biogenesis - correlation between structure and function. BBA, available on line, March 2012. |
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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