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Plant/Algal cell antibodies / Photosynthesis / LHC


Lhca5 | PSI type V chlorophyll a/b-binding protein

Art no: AS05 082
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product information

background  

Lhca5 is a low abundant chlorophyll a/b-binding protein that has been found to associate with Photoystem I in sub-stoechiometric amounts. Unlike the four main Lhca-types (Lhca1-4) Lhca5 seems to be able to associate with PSI or the LHCI at varying positions. The regulation pattern for the expressino of the Lhca5 gene differs from the standard Lhc pattern as expression seems to be positively regulated under light and cold stress conditions.

immunogen  

BSA-conjugated synthetic peptide derived from the Lhca4 protein of Arabidopsis thaliana (At1g45474). This sequence is highly conserved in Lhca5 proteins of angiosperms (monocots and dicots) and gymnosperms.

antibody format  

rabbit

polyclonal,

total IgG in PBS pH 7.4,

lyophilized

quantity  

200 µl

- for reconstitution add 200 µl of sterile water

storage  

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications  

western blot (WB)

related products  

AS01 005 Lhca1 | PSI type I chlorophyll a/b-binding protein

AS01 006 Lhca2 | PSI type II chloropyll a/b-binding protein

AS01 007 Lhca3 | PSI type III chlorophyll a/b-binding protein

AS01 008 Lhca4 | PSI type IV chlorophyll a/b-binding protein

LHC  available antibodies against pigment-binding proteins

additional information  

More information on the Lhca5 localization within the LHCI antenna can be found in Lucinski et al. (2006) Lhca5 interaction with plant photosystem I. FEBS Letters 580:6485-6488

application information

recommended dilution  

1: 1000- 1: 3000 with standard ECL (WB)

expected | apparent MW  

23 | 23 for Arabidopsis thaliana

confirmed reactivity  

Arabidopsis thaliana, Hordeum vulgare, Spinacia oleracea

predicted reactivity  

monocots and dicots

not reactive in  

no confirmed exceptions from predicted reactivity known in the moment

additional information  

to be added when available

selected references  

Ganeteg et al. (2005) Lhca5 – an LHC-Type Protein Associated with Photosystem I. Plant Mol Biol 54: 1573-5028



||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at  support@agrisera.com



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