Plant/Algal cell antibodies / Photosynthesis / LHC

product information

The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb2 is often coded by several nuclear genes and is found together with Lhcb1 within the mobile LHCII trimers involved in state1-state2 transition.

BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhb1 proteins from angiosperms (monocots and dicots) and gymnosperms, including Arabidopsis thaliana At2g05100 (b2.1), At2g05070 (b2.2), At3g27690 (b2.4) and also in a 4th isoform (b2.3, protein accession AF134124) listed by Jansson (1999) that is not annotated in the current TAIR database [2009-03-02].

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB), immunoprecipitation (IP)

AS01 002  Lhcb3 | LHCII type III chlorophyll a/b-binding protein

AS01 004  Lhcb1 | LHCII type II chlorophyll a/b-binding protein

LHC  available antibodies against pigment-binding proteins

A molecular characterisation of the LHCII proteins can be found in Caffarri et al. (2004) A Look within LHCII:  Differential Analysis of the Lhcb1−3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29): 9467–9476

application information

1 : 5000 with standard ECL (WB), 5 µl of antibody solution (IP)

25 | 25 kDa for Arabidopsis thaliana

Arabidopsis thaliana, Arachis hypogaea, Hordeum vulgare, Lycopersicon esculentum (Solanum lycopersicon), Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Spinacia oleracea, Zea mays, Physcomitrella patens and Chlamydomonas reinhardtii

angiosperms (monocots and dicots), gymnosperms, mosses, green algae

no confirmed exceptions from predicted reactivity known in the moment

Immunoprecipitation has been done using Immunoprecipitation kit from Roche, Cat.No. 11 719 386 001.

Rudowska et al. (2012). Chloroplast biogenesis - correlation between structure and function. BBA, available on line, March 2012.

Lang, E.G.E., S.J. Mueller, S.N.W. Hoernstein, J. Porankiewicz-Asplund, M. Vervliet-Scheebaum, R. Reski (2010). Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as basis for sub-cellular proteomics. Plant Cell Reports, DOI: 10.1007/s00299-010-0935-4. (open source)

Park et al. (2007) The Senescence-Induced Staygreen Protein Regulates Chlorophyll Degradation. Plant Cell 19:1649-1664