Plant/Algal cell antibodies / Photosynthesis / Other proteins

product information

Lci5 protein is encoded by the nuclear gene lci5, which is induced during the acclimation to low CO₂ conditions in the green alga Chlamydomonas reinhardtii. The Lci5 protein is peripherally associated with the stroma side of thylakoid membranes. In addition, this protein is heavily phosphorylated (7 sites, 3 Threonine and 4 serine residues, as shown by mass spec anlaysis) in cells growing under low CO₂ concentrations in the medium or during state transitions (state 2). Microarray analysis showed that the gene is up-regulated 3 times during the acclimation to low CO₂ conditions.

KLH-comjugated synthetic peptide chosen from a sequence of Chlamydomonas reinhardtii Lci5 protein A8IGD9

store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

western blot (WB), immunohistochenmistry (IHC)

collection of antibodies to Chlamydomonas reinhardtii proteins

antibody concentration is 0.9 µg/µl

application information

1: 2000 - 1: 5000 with standard ECL (WB)

32.7 kDa/24.1 kDa

Chlamydomonas reinhardtii

Chlamydomonas reinhardtii

no confirmed exceptions from predicted reactivity known in the moment

to be added when available

Turkina et al.  (2006). CO2 limitation induces specific redox-dependent protein phosphorylation in Chlamydomonas reinhardtii. Proteomics 6:2693-26704.