Plant/Algal cell antibodies / Photosynthesis / Protein standards-quantitation

product information

This product is a recombinant protein standard, source: Synechocystis strain PCC 6803.

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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS06 111 | anti-CP43' | IsiA homolog of plant CP43 antibodies

Collection of global antibodies

Collection of antibodies to photosynthetic proteins

antibodies are raised against highly conserved amino acid sequences in theIsiA protein. The IsiA protein standard can therefore be used in combination with anti-IsiA antibodies to quantitate IsiA from a range of cyanobacteria.

Quantitative western blot:  detailed method description.

application information

standard curve: 3 loads are recommended (2.5 and 10 μl).
For most applications a sample load of 0.2μg of chlorophyll will give a IsiA signal in this range.

positive control:a 2μl load per well is optimal for most chemiluminescent detection systems.

27 kDa (slightly larger than native protein due to His-tag)

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no confirmed exceptions from predicted reactivity known in the moment

Concentration: after adding 225  µl of milliQ water final concentration of the standard is 0.15 pmoles/µl

Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.

This standard is stabilized and does not require heating before loading on the gel.

to be added when available, standard released in February 2010