Plant/Algal cell antibodies / Photosynthesis / Protein standards-quantitation

product information

D2 protein (PsbD) forms the reaction core of PSII (Photosystem II) as a heterodimer with the D1 protein (PsbA). PsbD is homologous to the D1 protein, with slightly higher molecular mass of about 39,5 kDa. Accumulation of D2 protein is an important step in the assemply of the PSII reaction centre complex.
This product is a recombinant protein standard, source Synechocystis strain PCC 6803.

does not apply

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Western blot (WB)

collection of other protein standards

AS06 146 | anti-PsbD | D2 protein of PSII antibodies

collection of other global antibodies

collection of antibodies to PSII proteins

Global antibodies are raised against highly conserved amino acid sequences in the PsbD protein. The PsbD protein standard can therefore be used in combination with global anti-PsbD antibodies to quantitate PsbD from a wide range of species.

Quantitative western blot:  detailed method description.

application information

standard curve: 3 loads are recommended (0.5, 2 and 4μl).
For most applications a sample load of 0.2μg of chlorophyll will give a PsbD signal in this range.

positive control: a 2μl load per well is optimal for most chemiluminescent detection systems. 

This standard is stabilized and does not require heating before loading on the gel.

in most gel systems PsbD migrates around 28-30 kDa

does not apply

does not apply

no confirmed exceptions from predicted reactivity known in the moment

Concentration: after adding 225  µl of milliQ water final concentration of the standard is 0.25 pmoles/ul

Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.

to be added when available