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product information
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| background |
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Hcf101 is a novel protein required for photosystem I biogenesis. Hcf101-1 is a high-chlorophyll-fluorescence hcf) Arabidopsis thaliana mutant that lacks photosystem I. In green tissue the level of this protein is stimulated by light and the protein has not been detected in roots. It has been localized in chloroplast stroma. |
| immunogen |
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recombinant Arabidopsis thaliana Hcf101, amino acid residues 206-532 Q6STH5 |
| antibody format |
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rabbit |
polyclonal |
|
serum |
lyophilized |
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| quantity |
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200 µl |
for reconstitution add 200 µl of sterile water. |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB), immunolocalization (IL) |
| related products |
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AS03 037 | rabbit anti-RbcL stroma marker antibody |
| additional information |
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cellular [compartment marker] of plastid stroma |
application information
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| recommended dilution |
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1:3000 with standard ECL (WB), 1: 3000 (IL) |
| expected | apparent MW |
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57 | 50.5 kDa |
| confirmed reactivity |
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Arabidopsis thaliana |
| predicted reactivity |
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Arabidopsis thaliana |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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Immunolocalization has been done on isolated plastids, followed by separation into stroma and thylakoids |
| selected references |
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Stöckel et al. (2004) A Novel Protein for Photosystem I Biogenesis J. of Biol. Chem. Vol. 279, No. 11, Issue of March 12, pp. 10243–10251, 2004 |
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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