Plant/Algal cell antibodies / Photosynthesis / PSI (Photosystem I)

product information

PsaF (PSI-F) is a conserved subunit of type I photosynthetic reaction centers (Photosystem I, PSI). PSI is an integral membrane multi-protein complex that catalyzes the electron transfer from plastocyanin (or cytochrome c6) to ferredoxin (or flavodoxin). PsaF has been shown to be involved in the orientation of the soluble electron donor. In plants PSI-F is nuclear encoded and imported post-translationally into the chloroplast where it inserts into the thylakoid membrane.

KLH-conjugated synthetic peptide derived from the PsaF protein sequence of Arabidopsis thaliana (At1g31330). This peptide sequence is not completely conserved in mono- and dicots.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

PSI  available antibodies to Photosystem I proteins

Photosynthesis  available antibodies to photosynthetic proteins

to be added when available

application information

1:1000 with standard ECL (WB)

24 kDa | 17 kDa for Arabidopsis thaliana

Arabidopsis thaliana, Hordeum vulgare (very weak), Nicotiana tabaccum, Spinacia oleracea

Populus trichocarpa, Ricinus communis, Physcomitrella patens

Chlamydomonas reinhardtii, Synechococcus PCC 7942

to be added when available

Bock (2012). The plastid genome-encoded Ycf4 protein functions as a non-essential assembly factor for photosystem I in higher plants. Plant Physiol. ahead of print.

Fristedt et al. (2009). Phosphorylation of photosystem II controls functional macroscopic folding of photosynthetic membranes in Arabidopsis. Plant Cell 21:3950-3964

application example

2 µg of total leaf protein of Arabidopsis thaliana (1) and Hordeum vulgare (2) and total cellular protein of Chlamydomonas reinhardtii (3) and Synechococcus PCC 7942 (4) isolated with PEB (AS08 300) were separated on 4-12% Nupage Bis-Tris gels in in MES running buffer (Invitrogen) at 200V for 35 minutes. Proteins were transferred for 80 minutes at 30V to a PVDF membrane pre-wetted in methanol and equilibrated in 1X transfer buffer. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) and probed with anti-PsaF (AS06 104, 1:1000) and secondary HRP-conjugated goat anti-rabbit antibody (1:50 000, Abcam) for 1 hr in TBS-T containing 2% ECL Advance blocking reagent (GE Healthcare). Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signals was detected after 30 s using ECL Advance detection reagent (GE Healthcare) according to the manufacturers instructions and a CCD imager (FluorSMax, Bio-Rad).