Plant/Algal cell biology / Physcomitrella patens

product information

Chl27 catalyzes the cyclase reaction in chlorophyll biosynthesis.

residues 1-409 from Arabidopsis thaliana CHL27 fused to TrxA Q9M591

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS05 084 | anti-PsbA rabbit antibody, marker of thylakoid membrane

Collection of antibodies to Arabidopsis proteins

Collection of antibodies to Chlamydomonas proteins

[compartment marker] of chloroplast thylakoid and envelope membranes

application information

1:3000 with alkaline phosphatase (WB)

47 | 40 kDa (Arabidopsis thaliana)

Arabidosps thaliana, Horderum vulgare, Pisum sativum, Chlamydomonas reinhardtii, purple bacteria (CRD1) and (CHL27)

eudictos:Gossyium hirsutum; Euphorbia esula; Ricinus communis, monocots Hordeum vulgare, moss: Physcomitrella patens, cyanobacteria

no confirmed exceptions from predicted reactivity known in the moment

Antibodies detect two isoforms in Chlamydomonas reinhardtii, CRD1 in cells grown under copper deficiency (39.8 kDa) and CTH1 in cells grown with sufficient copper (40.7 kDa). Antibodies will also react with Arabidopsis thaliana, Hordeum vulgare, Pisum sativum, and purple bacteria

Moseley et al. (2002) Reciprocal expression of two candidate di-iron enzymes affecting photosystem I and light-harvesting complex accumulation. Plant Cell 14:673-688. Tottey et al. (2003) Arabidopsis CHL27, located in both envelope and thylakoid membranes, is required for the synthesis of protochlorophyllide. Proc. Natl. Acad. Sci. USA 100:16119-16124. Rzeznicka et al.  (2005) Xantha-l encodes one of at least three subunits of the aerobic Mg-protoporphyrin IX monomethyl ester cyclase in the chlorophyll biosynthetic pathway. Proc. Natl. Acad. Sci. USA 102: 5886-5891.