Plant/Algal cell antibodies / Physcomitrella patens

product information

PsaD (PSI-D) is a core subunit of photosystem I highly conserved in all photosynthetic organisms (including bacteria with Fe-S type reaction centers). In eukaryots its encoded by 1 to 2 nuclear gene(s) and imported as a precursor into the chloroplast. In the thylakoid membrane it associates with PsaA and PsaB on the stromal site of the PSI core forming the Fd-docking site. PsaD is also required for the stable assembly of PsaC.

KLH-conjugated synthetic peptide 100% conserved in all known plant PsaD sequences including Arabidopsis thaliana (At1g03130 and At4g02770) as well as Physcomitrella patens. The conservation in Chlamydomonas reinhardtii is high (14 of 16 aminoacids are identical).

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

PSI  available antibodies to Photosystem I proteins

Photosynthesis  available antibodies to photosynthetic proteins

PsaD has frequently been used as a marker for intact PSI reaction centers.

application information

1 : 1000 with SuperSignal West Pico detection (WB)

17.9 | 20 (for Arabidopsis thaliana)

dicots: Arabidopsis thaliana, Spinacia oleracea, monocots: Hordeum vulgare, Oryza sativa, Zea mays, moss: Physcomitrella patens, algae: Chlamydomonas reinhardtii

plants (monocots, dicots and conifers), green algae

Synechococcus elongatus sp. PCC 7942

this antibody is a replacement for former product, anti-PsaD AS04 046

Yadavalli et al. (2012). Differential degradation of photosystem I subunits under iron deficiency in rice. J Plant Physiol. March 22.

Yamamuchi et al. (2011). Plants switch photosystem at high temperature to protect photosystem II. Nature Proceedings in press.

Lang, E.G.E., S.J. Mueller, S.N.W. Hoernstein, J. Porankiewicz-Asplund, M. Vervliet-Scheebaum, R. Reski (2010). Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as basis for sub-cellular proteomics. Plant Cell Reports, DOI: 10.1007/s00299-010-0935-4. (open source)

application example

10 µg of total leaf protein extracted with PEB (AS08 300) from (1) Zea mays, (2) Chlamydomonas reinhardtii, and (3) Spinacia oleracea were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 80 min (30V) to nitrocellulose. Filter was blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-PsaD (AS09 461, 1:1000, 1h) and secondary anti-rabbit (1:40000, 1h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with SuperSignal West Pico (Thermo Scientific) using a GenoPlex Chemi CCD (accumulated signal 10 x 30s exposure, bin 2x2).