Plant/Algal cell antibodies / Physcomitrella patens

product information

PsbW is a nuclear-encoded protein located in the thylakoid membrane of the chloroplast. It is a core component of Photosystem II. Altrnative name: PSII 6.1 kDa protein

KLH-conjugated synthetic peptide derived from PsbW protein sequence of Arabidopsis thaliana At2g30570

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

collection of antibodies to PSII proteins

to be added when available

application information

1:15 000 with standard ECL (WB)

13.6 | 6.1 kDa

Arabidopsis thaliana, Horderum vulgare, Nicotiana tabacum, Spinacia oleracea

dicots including Vitis vinifera, monocots including Oryza sativa, Zea mays, moss Physcomitrella patens

Chlamydomonas reinhardtii, Synechococcus sp. PCC 7942

not available at the moment

Suorosa et al. (2006). PsbR, a missing link in the assembly of the oxygen-evolving complex of plant photosystem II. J. Biol. Chem. 1: 145-150. Garcia-Cerdan et al. (2008). Antisense inhibition of the PsbX protein affects PSII integrity in the higher plant Arabidopsis thaliana. Plant Cell Physiol. 2: 191-202.

application example

2 µg of total protein from (1) Arabidopsis thaliana leaf, (2) Horderum vulgare leaf ), (3) Chlamydomonas reinhardtii total cell , (4) Synechococcus sp. 7942 total cell were all extracted with PEB (AS08 300) and separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 50 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).  Exposure time was 1 second.