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product information
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| background |
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Catalase is an enzyme found in most living organisms which is catalazying decomposition of hydrogen peroxide to water and oxygen. In plant cells catalase is found in peroxisomes. This enzyme is involved in photorespiration and symbiotic nitrogen fixation. |
| immunogen |
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KLH-conjugated peptide chosen from know plant catalase sequences including Arabidopsis thaliana |
| antibody format |
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| quantity |
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| storage |
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store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB) |
| related products |
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AS08 374 | anti-KatG | catalase peroxidase (HPI), cyanobacterial |
| additional information |
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This antibody is also recognizing Arabidopsis thaliana AtCAT2 isoform.
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application information
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| recommended dilution |
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1: 1000 with standard ECL (WB) |
| expected | apparent MW |
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57 | 55 kDa |
| confirmed reactivity |
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Arabidopsis thaliana, Brassica oleracea, Hordeum vulgare, Nicotiana bentamina, Oryza sativa, Solanum lycopersicum, Spinacia oleracea, Zea mays
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| predicted reactivity |
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dicots including: Gossypium mexicanum, Vitis vinifera, trees: Populus jackii
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| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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To obtain reactivity with Solanum lycopersicum urea gel needs to be apply. Please, contact us for more details. |
| selected references |
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Ray et al. (2011). Expression and purification of soluble bio-active rice plant catalase-A from recombinant Escherichia coli. J. Biotechnology |
10 µg of total protein from Arabidopsis thaliana Col0 (1), Cat2-(Col0) (2), Ler0 (3), Cat2-(Ler0) (4), Zea mays (5), Oryza sativa (6), Brassica oleracea (7), Nicotiana bentamina (8) were extracted with 60mM Tris pH 6.9, 10mM DTT, 20% glycerol, 1mm PMSF were separated on 12.5% SDS-PAGE and blotted 1h to PVDF. Blot was blocked with 3% skim milk in PBS+0.05% Tween20 for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation in the same buffer. The antibody solution was decanted and the blot was rinsed briefly three times, then washed 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, Agrisera, AS09 602) diluted to 1:50 000 in 3% skim milk in PBS+0.05% Tween20 for 1h at RT with agitation. The blot was washed as above and developed for 1 min with Western Lightning Plus-ECL ( PerkinElmer )according to the manufacturers instructions. Exposure time was 5min. in ChemiDoc XRS+ (Biorad ).
Courtesy of Brigitte van de Cotte, Gent University, Belgium
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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