|
product information
|
| background |
|
ClpC is a chloroplastic protein of the Hsp100 family. It is believed to function as a housekeeping enzyme, both in its capacity as an independent molecular chaperone and as the regulatory component of the Clp protease. |
| immunogen |
|
recombinant ClpC (C-terminal domain overexpressed as fusion with maltose-binding protein) Q55023 |
| antibody format |
|
rabbit |
polyclonal |
|
serum |
lyophilized |
|
| quantity |
|
100 µl, |
for reconstitution add 100 µl of sterile water. |
|
| storage |
|
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
|
western blot (WB); immunoprecipitation (IP); immunohistochemistry (IHC) |
| related products |
|
AS08 344 | ClpB1 ATP-dependent chaperone AS08 355 | ClpB2 ATP-dependent chaperone |
| additional information |
|
Anti-ClpC antibodies will also recognized Arabidopsis thaliana isoform ClpC1 (At5g50920) and ClpC2 (At3g48870). |
application information
|
| recommended dilution |
|
1 : 5000 on 10 µg of total protein, detected with standard ECL (WB); 1: 1000 (IHC) |
| expected | apparent MW |
|
n.a. | 87 kDa |
| confirmed reactivity |
|
Arabidopsis thaliana, Synechococcus sp., Chlamydomonas reinhardtii, Streptomycessp. |
| predicted reactivity |
|
dicots including: Nicotiana tabacum, Pisum sativum, Solanum tuberosum, monocots including: Oryza sativa, Zea mays, trees. Populus trichocarpa, moss: Physcomitrella patens, red alga, Ostreococcus sp. |
| not reactive in |
|
different strains of Mycobacterium smegmatis |
| additional information |
|
n.a. |
| selected references |
|
Porankiewicz & Clarke (1997) Induction of the heat shock protein ClpB affects cold acclimation in the cyanobacterium Synechococcs sp. strain PCC7942. J Bacteriol 179:5111-5117; Karradt et al. (2008) NblA, a Key Protein of Phycobilisome Degradation, Interacts with ClpC, a HSP100 Chaperone Partner of a Cyanobacterial Clp Protease. J Biol Chem 283: 32394-32403 |
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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