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product information
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| background |
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Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H202,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD |
| immunogen |
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15 amino acids synthetic peptide designed from olive pollen cytosolic Cu/Zn cDNA consensus sequences (GenBank Accessions EU250757 to EU250797), UniProt Q8L5E0 |
| antibody format |
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hen |
polyclonal |
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total IgY in 10mM Tris, 150 mM NaCl, 0.02% sodium azide |
liquid |
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| quantity |
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| storage |
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store at -4°C. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB) |
| related products |
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AS06 170 | anti-CSD2 | chloroplastic Cu/Zn superoxide dismutase AS06 125 | anti-FeSOD | chloroplastic Fe-dependent superoxid dismutase AS07 219 | anti-CCS | chloroplastic copper chaperone for SOD AS09 524 | anti-MnSOD | manganese superoxide dismutase collection of antibodies to plant stress proteins |
| additional information |
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Total IgY concentration is 2.3 mg/ml Reaction of the antibody to chloroplastic SOD isoform has not been determined yet |
application information
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| recommended dilution |
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1 : 1500 with Alexa 488 (WB) |
| expected | apparent MW |
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15.3 | 16 kDa (Olea europaea L.) |
| confirmed reactivity |
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Arabidopsis thaliana (leaves), Olea europaea (pollen), Spinacia oleracea (leaves) |
| predicted reactivity |
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dicots inlcuding: Ananas ananas, Herlianthus sp. , Plantago major, Solidago sp. trees: Betula pendula |
| not reactive in |
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Nicotiana tabacum |
| additional information |
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Note: Antibody recognizes two to three isoforms of Cu/Zn SOD in olive pollen depending on the olive cultivar. |
| selected references |
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Alché, J.D., Corpas, F.J., Rodríguez-García, M.I. and del Río, L.A.: "Identification and immunolocalization of superoxide dismutase isoenzymes of olive pollen". Physiol. Plantarum 104, 772-776. 1998. |
application example
25 µg of total protein from Olea europaea pollen were separated on 12% acrylamide gels and blotted onto PVDF. Membranes were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-Cu/Zn SOD (AS10 652, 1:1500, overnight, 4º C) and secondary antibody anti-chicken IgG conjugated to Alexa 488 (1:2000, 1 h) (Molecular Probes, Invitrogen). Signal was detected with a PharosSX fluorescence scanner (Bio-Rad).
The antibody recognizes a protein of c.a. 16 kDa. and another band likely corresponding to the protein dimer.
Courtesy Dr. Juan de Dios Alche, CSIC, Spain | .jpg) |
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