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product information
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| background |
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HDT3 is probably mediating in the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. This protein is also involved in the modulation of abscisic acid and stress-responsive genes.Synonymes:HD-tuins protein 3, Histone deacetylase 2c |
| immunogen |
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recombinant part of Arabidopsis thaliana HDT3 Q9LZR5 |
| antibody format |
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rabbit |
polyclonal |
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affinity purified serum |
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| quantity |
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| storage |
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store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB), immunoprecipitation (IP) |
| related products |
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| additional information |
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antibody is present in PBS + 50 % glycerol and 0.01 % of sodium azide as preservative of bacterial growth |
application information
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| recommended dilution |
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1 : 2 500 with standard ECL (WB) |
| expected | apparent MW |
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31.8 | 40 kDa |
| confirmed reactivity |
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Arabidopsis thaliana |
| predicted reactivity |
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Glycine max, Populus trichocarpa, Ricinus communis, Solanum tuberosum |
| not reactive in |
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no confirmed exceptions from predicted reactivity are currently known |
| additional information |
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to be added when available |
| selected references |
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to be added when available, antibody released in May 2011 |
application example
50 µg of total protein from Arabidopsis thaliana nuclei extracted with Nuclei Lysis Buffer (1% SDS, 50mM Tris-HCl, 10mM EDTA, Complete EDTA-free) were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (WestranS 0,20 µm, Whatman).HD2C+/- heterozygote nuclear extract (1), HD2C -/- homozygote nuclear extract (2), HD2C -/- homozygote nuclear extract (3), HD2C -/- homozygote nuclear extract (4), 35S:HD2C-GFP nuclear extract (5), 35S: HD2C-GFP nuclear extract (6), Arabidopsis thaliana Col-0 nuclear extract (7), Arabidopsis thaliana Col-0 nuclear extract (8). Blots were blocked with 5% fat free milk in TBST for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 500 in 2,5% fat-free milk in TBST overnight at 40C with agitation. The antibody solution was decanted and the blot was washed with TBST three times for 10 min and blocked in 10% fat-free milk in TBST for 10 min. Next, blot was incubated in secondary antibody diluted to 1:5 000 in 5% fat-free milk in TBST for 2h at RT with agitation. The blot was washed six times for 10 min with TBST and developed for 5 min with ECL+ (Amersham) according to the manufacturers instructions. Exposure time was 30 seconds. Note: Additional band in lanes 5 and 6 comes from HD2C-GFP. Courtesy Daniel Buszewicz, University of Warsaw, Poland
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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