HSP90-2 (heat shock protein 90-2) is a constitutive isoform of HSP90.
immunogen
full length recombinant Hsp90-2 from Arabidopsis thaliana
antibody format
rabbit;
polyclonal;
serum;
lyophilized
quantity
100 µl
- for reconstitution add 100 µl of sterile water
storage
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
no confirmed exceptions from predicted reactivity known in the moment
additional information
to be added when available
selected references
to be added when available, antibody released in June 2011.
application example
5 µg total protein extracted from 15 day old Arabidopsis thaliana seedlings that were either kept at 22ºC (C) or heat treated at 38ºC for 2 hours prior to protein extraction (HS). As positive control 10 ng of recombinant Hsp90-2 or Hsp90-1 were separated side by side with the plant samples on 10-15 % gradient SDS-PAGE and blotted to nitrocellulose (Bio-rad). Blots were blocked following transfer with 5% low fat milk in low salt buffer for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 3000 for 2h at room temperature with agitation in the blocking solution. The primary antibody solution was removed and the blot was rinsed briefly twice, then washed 4 times for 15 min each at room temperature with agitation using low salt buffer. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxides conjugated, from Agrisera, AS09 602), diluted to 1:35 000 for 1h at room temperature with agitation then washed as above and treated with ECL detection reagent according to the manufacturer's instructions. Exposure time was 20 seconds.
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