Plant/Algal cell antibodies / Populus sp.

product information

Plasma membrane aquaporin, PIP2;7 is water channel protein required for water transport across cell membrane. Alternative names:  plasma membrane intrinsic protein 2-7, AtPIP2;7, plasma membrane intrinsic protein 3, salt stress-induced major intrinsic protein, PIP3a

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PIP2;7,P93004

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

ELISA (ELISA), western blot (WB)

AS09 488 | PIP2;1 | aquaporin PIP2;1

AS09 490 | PIP2;2 | plasma membrane aquaporin 2b

AS09 491 | PIP2;1,PIP2;2,PIP2;3 |plasma membrane intrinistic protein 2-1,2-2, 2-3

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of plasma membranes are used, one µg load per well should be sufficient.

Protocol for isolation of plant plasma membrane proteins can be found here.

This antibody has a potential to work in immunolocalization studies, as it is recognizing N-terminall part of the sequence, which is exposed to the cytoplasm.

application information

1: 8000 (ELISA), 1: 2000 with standard ECL (WB)

29.7 | 30.5 kDa (Arabidopsis thaliana)

Arabidopsis thaliana

dictos including: Brassica oleracea, Spinacia oleracea, Vitis vinifera,trees: Juglans regia, Populus tremulax Populus tremuloides

no confirmed exceptions from predicted reactivity known in the moment

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it  contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Manufactured by Operon Biotechnologies.

to be added when available

application example

1 µg and 10 µg of crude membrane fraction/lane from Arabidopsis thaliana  were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-PIP2;7 antibodies (AS09 469, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.