|
product information
|
| background |
|
Phospholipase D (EC=3.1.4.4) is an enzyme involved in lipid degradation by hydrolyzing glycerol phospholipids. Involved in many cellular process including phytohormone action, membrane deterioration, senescence and more. Alternative name: phosphatidylcholine phosphatido-hydrolase. |
| immunogen |
|
phospholipase D isolated and purified from Brassica oleracea |
| antibody format |
|
rabbit |
polyclonal |
|
affinity purified IgG in phosphate buffer saline, |
lyophilized |
|
| quantity |
|
1 mg |
for reconstitution please add 0.5 ml of sterile destilled water. |
|
| storage |
|
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
|
western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC) |
| related products |
|
AS09 556 | anti-PLD phospholipase D AS09 557 | anti-PLD phospholipase D (biotinylated) |
| additional information |
|
antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition. |
application information
|
| recommended dilution |
|
1: 100 - 1: 1000 for techniques listed above |
| expected | apparent MW |
|
92 kDa |
| confirmed reactivity |
|
Brassica oleracea |
| predicted reactivity |
|
dicots including: Arabidopsis thaliana, Cucumis melo, Ricinus communis, trees: Populus balsamifera |
| not reactive in |
|
no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
|
Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran. |
| selected references |
|
to be added when available |
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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