5 µg of BBY particles (
Pisum sativum) (lane 1),
Arabidopsis thaliana photosystem I preparation (lane 2),
Arabidopsis thaliana thylakoid preparation (lane 3),
Arabidopsis thaliana chloroplast preparation (4) was separated on
4-12% NuPage (Invitrogen) and blotted on
nitrocellulose. Filters where blocked (1h), incubated with
1: 1000 anti-PsaE (1h) followed by incubation with
1: 20 000 secondary anti-rabbit (1h) coupled to HRP and visualization with standard ECL (GE Healthcare). Isolation of total leaf protein was done with PEB (see product
AS08 300).